Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG.No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Sterile-filtered liquid

Storage: Store at 2-8°C. Prepare working dilution on day of use. Expiration date: one year from date of receipt. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 2.0 mM Sodium Borate, pH 9.0 Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:1:10 - 1:100Conjugate

40 nm Colloidal Gold

Colloidal gold conjugates are widely used commercially in lateral flow immunoassays due to their ease of production, conjugate stability and intense optical properties that generate highly sensitive tests with results visible to the naked eye.Conjugates are prepared by passive absorption of the antibody to the gold particle, and are sterile filtered in a low ionic strength buffer containing a preservative.

Images & References

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This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

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