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当前位置: 首页 > 产品中心 > Goat_resistance > 杰克逊/西™5亲合驴抗山羊IgG(H+L)/0.5 mg/705-175-147
商品详细杰克逊/西™5亲合驴抗山羊IgG(H+L)/0.5 mg/705-175-147
杰克逊/西™5亲合驴抗山羊IgG(H+L)/0.5 mg/705-175-147
杰克逊/西™5亲合驴抗山羊IgG(H+L)/0.5 mg/705-175-147
商品编号: 705-175-147
市场价: ¥2900.00
美元价: 1087.50
产地: 美国(厂家直采)
公司:
产品分类: 抗山羊
公司分类: Goat_resistance
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Information Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule goat IgG.It also reacts with the light chains of other goat immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, syrian hamster, horse, human, mouse, rabbit and rat serum proteins, but it may cross-react with immunoglobulins from other species.

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Usage Physical State: Freeze-dried solid

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Stabilizer:15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:

1:100 - 1:400 for most applicationsDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Conjugate

Cyanine Cy™5

Amax: 650Emax: 670nm

Cy5 conjugates are excited maximally at 650 nm and fluoresce maximally at 670 nm. They can be excited to about 98% of maximum with a krypton/argon laser (647 nm line) or to about 63% of maximum with a helium/neon laser (633 nm line). Cy5 can be used with a variety of other fluorophores for multiple labeling due to a wide separation of its emission from that of shorter wavelength-emitting fluorophores. However, we recommend Alexa Fluor® 647 as the preferred far-red-fluorescing dye because it is brighter than Cy5 in aqueous mounting media. The reverse is true when mounting in non-polar, plastic mounting media, i.e. Cy5 is brighter than other far-red-fluorescing dyes, including Alexa Fluor® 647 and DyLight 650.

Images & References

Skin sweat gland nerve fibers labeled with rabbit anti-PGP9.5 + donkey anti-rabbit Cy2 (711-225-152 green), secretory epithelial cells labeled with rat anti-CD44 + donkey anti-rat Cy3 (712-166-150 red) and basement membranes labeled with goat anti-collagen IV + donkey anti-goat Cy5 (705-175-147 blue). Brian McAdams and William Kennedy, University of Minnesota.


This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Cy is a trademark of GE Healthcare Bio-Sciences Ltd. Jackson ImmunoResearch is licensed by GE Healthcare Bio-Sciences Ltd. to manufacture and sell conjugates of Cy2, Cy3, and Cy5 reactive dyes under US Patent Number 5,268,486, and other patents pending.

品牌介绍
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