Jackson/Biotin-SP (long spacer) AffiniPure Goat Anti-Swine IgG (H+L)/2.0 ml/114-065-003

来自 : 发布时间：2024-04-27

Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule swine IgG.It also reacts with the light chains of other swine immunoglobulins.No antibody was detected against non-immunoglobulin serum proteins.The antibody may cross-react with immunoglobulins from other species.Physical State: Freeze-dried solid Storage:

Store freeze-dried powder at 2-8°C. When ready to use, rehydrate with indicated volume of d. water and centrifuge if not clear. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Note: after the addition of glycerol, the concentration of protein and buffer salts is one-half of the original. Alternatively, aliquot and freeze the product at -70°C or below in the absence of glycerol. Avoid repeated freezing and thawing.Expiration date: one year from date of rehydration. However, the expiration date may be extended if the product is stored according to the recommendation and the test results are acceptable for its intended use.

Purity:The antibody was purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 Preservative:0.05% Sodium Azide Suggested Working Concentration or Dilution Range:Using Fluorophore Conjugated Streptavidin:Histo-/Cyto-chemistry:- 1:200-1:1,000Flow Cytometry:- 1:200-1:1,000Using Enzyme Conjugated Streptavidin:ELISA:- 1:20,000-1:400,000Western Blots: 1:20,000-1:400,000Histo-/Cyto-chemistry:- 1:500-1:5,000Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Biotin-SP (long spacer)

Biotin-SP is our trade name for biotin with a 6-atom spacer positioned between biotin and the protein to which it is conjugated. When Biotin-SP-conjugated antibodies are used in enzyme immunoassays, there is an increase in sensitivity compared to biotin-conjugated antibodies without the spacer. This is especially notable when Biotin-SP conjugated antibodies are used with alkaline phosphatase-conjugated streptavidin. Apparently, the long spacer extends the biotin moiety away from the antibody surface, making it more accessible to binding sites on streptavidin. Biotinylated antibodies require an additional reagent for visualization. We offer streptavidin and Mouse Anti-Biotin conjugated to fluorophores and enzymes.